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. 2019 May 30;12(5):dmm037887. doi: 10.1242/dmm.037887

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Effect of glucocorticoids on macrophage and neutrophil recruitment upon tail amputation in Tg(mpx:GFP/mpeg1:mCherry-F) larvae. (A) Schematic drawing of a zebrafish larva at 3 dpf. The red line shows the site of amputation. The black dashed box shows the area in which cells were counted to quantitate the recruitment. (B) The number of macrophages recruited to the wounded area at 4 hpa. In the beclomethasone (Beclo), dexamethasone (Dexa) and prednisolone (Pred) groups, no significant differences were observed compared the vehicle-treated (control) group. In the hydrocortisone (Hyd) group, a significantly decreased number of macrophages was observed. (C) The number of neutrophils recruited to the wounded area at 4 hpa. For all glucocorticoid-treated groups, a significantly reduced number of neutrophils was recruited compared to the control group. Data are mean±s.e.m. from three independent experiments. *P<0.05; ***P<0.001; ****P<0.0001 (determined using ANOVA). ns, non-significant.