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. 2019 May 30;12(5):dmm038513. doi: 10.1242/dmm.038513

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Increased expression of Enpp1 and Ank and elevated PPi concentration in the mandible of Fgfr2^+/S252W embryos at E16.5. (A-H) RNA expression of Enpp1 and Ank in the mandible of Fgfr2^+/+ littermate (A,B,E,F) and Fgfr2^+/S252W embryos (C,D,G,H) was validated using ISH. B,D,F and H show higher magnification of the boxed areas in A,C,E and G, respectively. The weight of the mandible (I) and PPi concentration in the mandible (J) were measured for Fgfr2^+/+ littermates (n=7) and Fgfr2^+/S252W (n=11) embryos at E16.5. Data are mean±s.e.m. *P<0.05, two-tailed Welch's t-test. Scale bars: 100 µm.