Fig. 4.

Differential chromatin accessibility in progenitor Six2^GFP(high) (P0-H) and differentiating Six2^GFP(low) (P0-L) NPC. Heatmap (A), Principal Component Analysis (B) and MA plot (C) of ATAC-seq peaks in P0-H versus P0-L NPC. (D) Heatmap of ATAC peaks in the −1 to +1 kb around TSS in annotated genes at P0-H and P0-L NPC. (E) Heatmap showing differential chromatin accessibility of selected differentiation genes in P0-H versus P0-L NPC. Red represents gain, whereas blue represents loss of open chromatin regions. (F,G) ATAC-seq tracks comparing P0-H and P0-L NPC in the HNF1b and NCam1 loci. Grey colored boxes highlight gain of open chromatin in P0-L versus P0-H. (H) GO functional annotation of ATAC peaks by GREAT analysis in P0-H and P0-L NPC.