Fig. 7.

ChIP-seq profiling of histone modifications in E13 and P0 NPC reveal active enhancer gain with age. (A) (top) Venn diagrams of unique and shared H3K4me1 and H3K27ac active regions (top); (bottom) affinity binding analysis performed on the shared regions reveals a net gain of active H3K27-marked enhancers in P0 NPC. (B) GREAT analysis of the unique active enhancers in E13 versus P0 NPC reveals age-dependent biological processes. (C) HOMER analysis at E13 and P0 active enhancers defines distinct sets of enriched motifs in E13 and P0 NPC and reveals age-related gain of Bach2 and AP1 motifs.