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. 2019 Apr 29;8(5):bio042341. doi: 10.1242/bio.042341

Fig. 1.

Fig. 1.

Numb impairs ductal elongation in the pubertal mouse mammary gland. (A) Fluorescence images of mammary ducts immunostained for Numb (magenta) in control and Numb-deficient ducts. (B) Wholemount images of control and Numb-deficient glands from four-, six- and 12-week-old mice. (C) Diagram describing growth measurements relative to the distal end of the lymph node (black dashed line, reference position). The white dashed line indicates the distal tip of the ductal tree. (D) Scatter plot of ductal outgrowth in reference to the lymph node in four-, six- and 12-week-old mice. Positive values represent growth past the distal edge of the lymph node, whereas negative values indicate ducts that have not passed the lymph node. n=7–15 glands for each group (one-way ANOVA). (E) Scatter plot of mammary gland branching. n=9–10 glands for each group. (F) Scatter plot of Ki67-positive cells in ducts. n=17–22 ducts from four glands for each group. (G) Scatter plot of cleaved-Caspase-3-positive cells in ducts. n=21 ducts from three to four glands for each group. Error bars=s.e.m. Scale bars: A, 20 μm; B, 2 mm. Unpaired t-test; two-tailed: ****P<0.0001; *P<0.05; ns, not significant.