Fig. 4.
Numb affects luminal cell shape in pubertal mammary glands. (A) Diagram of a duct showing measurement parameters: Clumen represents the inner circumference of the lumen; Cduct represents the outer circumference of the duct; dark blue represents the cross-sectional face of the duct; light blue represents the cell length along the duct. Red represents the outline of a luminal cell. (B) Scatter plot of lumen size (Clumen) for control and Numb-depleted mammary ducts. (C) Scatter plot of duct size (Cduct) for control and Numb-depleted mammary ducts. (D) Fluorescence images of luminal (red) and myoepithelial (Myo; green) cell markers in control and Numb-deficient ducts. Scale bar: 20 μm. (E) Scatter plot of luminal duct cell areas. Color shading on the scatter plots shows the density distribution of data. (F) Scatter plot of luminal cell perimeters. (G) Scatter plot of luminal cell shapes. Shape factor varies from circular (0) to elongated polygon (1.0). (H) Scatter plot of end bud body cell areas. (I) Scatter plot of end bud body cell perimeters. (J) Scatter plot of end bud body cell shapes. (K) Scatter plot of cell packing density (number of cells/unit length) for luminal duct cells. (L) Scatter plot of cell packing density for myoepithelial (Myo) duct cells. (M) Scatter plot of cell packing density for end bud body cells. n=12–14 ducts over four glands for each group. (N) Scatter plot showing calculated average ductal cell volumes. n=14 cells over four to five glands for each group. (O) Scatter plot showing calculated relative volume of mammary ducts. (P) Scatter plot showing calculated total number of cells per duct. Error bars=s.e.m. Unpaired t-test; two-tailed: ****P<0.0001, ***P<0.0005, **P<0.01, *P<0.05, ns, not significant. n=9–11 ducts from five to six glands for each group (B,C); n=43–66 cells from three glands for each group (E–G); n=187–216 cells from four glands for each group (H–J); n=15 ducts over five glands for each group (K,L); n=7–9 ducts over seven to nine glands for each group (O,P).