Skip to main content
. 2019 Apr 29;8(5):bio042341. doi: 10.1242/bio.042341

Fig. 7.

Fig. 7.

Numb is not required for epithelial cell migration during organoid morphogenesis. (A) DIC images of control and Numb-deficient organoids after 3 days in culture. (B) Fluorescence images of sections of control and Numb-deficient organoids immunostained for CK8 and CK14 and label luminal and myoepithelial lineages. (C) DIC images of control and Numb-deficient organoids. Numbers and the percentage of organoids showing surface disorganization (see arrowheads). (D) Scatter plot of average cell velocity in control and Numb-deficient organoids. (E) Scatter plot of cell migration directionality (ratio of total distance over displacement). (F) Scatter plot of total organoid area from control and Numb-deficient organoids. n=13–15 organoids from both groups. (G) Scatter plot of cell packing density measured from internuclear distances of cells in control and Numb-deficient organoids. n=46–54 neighboring cell pairs from five to 11 organoids from both groups. n=100–215 cells from nine to 13 organoids from both groups (D,E). Error bars=s.e.m. Scale bars: 50 μm; Unpaired t-test; two-tailed: **P<0.01; ns, not significant.