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. 2019 Apr 16;8(5):bio040840. doi: 10.1242/bio.040840

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Promotion of Hh signaling by downregulated Ptch1 gene suppressed autophagy level. Embryos were injected with ptch1MO and ptch1Mis at the 1–4 cell stage and larvae were collected at 6 dpf for qPCR and western blot. GAPDH as a loading control. The means and s.d. were derived from triple duplicates. (A,B) SHH protein and autophagy-related proteins were detected by western blot. (C) Ptch1MO-injected group compared with WT group and ptch1Mis-injected group. Autophagy gene mRNA levels were tested by qPCR. *P<0.05, **P<0.01 versus WT groups (one-way ANOVA).