Fig. 7.

Voluntary exercise increases ΔFosB/FosB expression in the LDT/PPTg neurons. Double immunostaining with nNOS and ΔFosB/FosB antibodies was performed on the brain stem sections containing LDT/PPTg. The locations of the LDT and PPTg containing nNOS + (cholinergic) neurons in the midbrain are indicated as squares in (A). Aq: aqueduct. (B) Mouse brain atlas showing areas of LDT/PPTg (blue squares). No ΔFosB/FosB is expressed in LDT (C, D, E)/PPTg (F, G, H) neurons in Sham-Sedentary mice, while numerous ΔFosB/FosB positive nuclei were observed in both nNOS + (arrowheads) and nNOS- neurons in the LDT (I, J, K)/PPTg (L, M, N) in Sham-Runner mice. Bars = 50 μm.