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. Author manuscript; available in PMC: 2019 Jun 5.
Published in final edited form as: Adv Exp Med Biol. 2017;953:209–306. doi: 10.1007/978-3-319-46095-6_6

Fig. 4.

Fig. 4

Dorsal determinant transport in zebrafish. (a) Sequence of events in wildtype embryos. RNAs and other dorsal determinants are localized vegetally during oogenesis (blue). After fertilization, cytoplasm streams to the animal pole, forming the future blastoderm. Microtubule assembly initiates ~20 min post-fertilization at the vegetal pole of the yolk cell; localized RNAs and Syntabulin protein (Sybu) are shifted toward the future dorsal side. Microtubule networks in the lateral cortex facilitate global transport animal-ward, which on the dorsal side would contain axis determinants. (b) In hecate (hec) mutants lacking Grip2a, maternal vegetal localization occurs, but cortical rotation and microtubule assembly are deficient post-fertilization. This image is reproduced and modified from, Ge X, Grotjahn D, Welch E, Lyman-Gingerich J, Holguin C, Dimitrova E, et al. (2014) Hecate/Grip2a acts to reorganize the cytoskeleton in the symmetry-breaking event of embryonic axis induction. PLoS Genet 10(6): e1004422. doi:10.1371/journal.pgen.1004422, under the terms of the Creative Commons Attribution License (CC BY 4.0)