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Determination of Michaelis-Menten affinity constants (KM) for biotransformation of phenanthrene (panel A), pyrene (panel B), and benzo[a]pyrene (panel C) by trout liver S9 fractions. Solid lines were obtained by fitting Equation 4 to initial depletion rates (kdep) determined at different substrate concentrations. Each point represents the mean ± SD of kdep values from triplicate depletion assays.
