Table 1.
Characterization of pooled trout liver S9 fractions used in various experiments
| Experiments performed using each pool of S9 proteina | ||||
|---|---|---|---|---|
| Pool 1: Effect of incubation time |
Pool 2: Protein dependence and effect of alamethicin |
Pool 3: Substrate concentration dependence and effect of acetone |
Pool 4: Chemical binding |
|
| Fish number | 2♂, 4♀ | 1♂, 4♀ | 4♂, 1♀ | 3♂, 3♀ |
| Fish body wt. (g) | 349 ± 50 | 382 ± 39 | 420 ± 67 | 315 ± 46 |
| HSI (%) | 0.98 ± 0.14 | 1.11 ± 0.06 | 0.92 ± 0.10 | 1.01 ± 0.09 |
| GSI, males (%) | 0.04 ± 0.01 | 0.03 | 0.04 ± 0.01 | 0.05 ± 0.02 |
| GSI, females (%) | 0.16 ± 0.08 | 0.19 ± 0.11 | 0.22 | 0.19 ± 0.07 |
| Protein content (mg/mL) | 23.9 ± 0.04 (4) | 24.2 ± 0.8 (4) | 25.6 ± 0.9 (4) | 24.1 ± 0.1 (3) |
| CYP content (pmol/g liver) | 4351 ±232 (4) | 3771±280 (4) | 3755 ± 256 (4) | N/P |
| EROD activity (pmol/min/mg protein) | 4.5 ± 0.2 (4) | 5.7 ± 0.3 (4) | 6.0 ± 0.3 (4) | 6.1 ± 0.2 (3) |
| UGT activity (pmol/min/mg protein) |
978 ± 46 (4) | 1074 ± 36 (4) | 678 ± 115 (4) | 804 ± 25 (4) |
| GST activity (nmol/min/mg protein) |
538 ± 14 (4) | 698 ± 36 (4) | 466 ± 9 (4) | 553 ± 47 (3) |
Abbreviations: HSI – hepatosomatic index; GSI – gonadosomatic index; EROD – 7-ethoxyresorufin-O-deethylase; UGT – UDP-glucuronosyltransferase; GST – glutathione-S-transferase; CYP – cytochrome P450; N/P – not performed
a
Values given in parentheses indicate the number of replicated determinations.