Table 2.

Fitted depletion rate constants (k_dep; 1/h) from S9 optimization studies

	Phenanthrene	Pyrene	Benzo[a]pyrene
Incubation timea
0 h	1.76 ± 0.03	23.00 ± 0.99	36.58 ± 0.62
0.5 h	-----	18.00 ± 0.54	33.97 ± 1.22
1 h	1.24 ± 0.00	14.47 ± 0.09	29.59 ± 0.67
2 h	0.82 ± 0.01	9.88 ± 0.49	23.91 ± 0.40
3 h	0.63 ± 0.01	7.07 ± 0.40	17.95 ± 0.78
Acetone spiking solventb,c
0.25%	1.73 ± 0.05d
0.5%	1.77 ± 0.02d
1.0%	1.49 ± 0.04e
2.0%	0.91 ± 0.03f
5.0%	0.36 ± 0.02g
Alamethicin/methanolb
0 μg/mL/0%	2.72 ± 0.02
0 μg/mL/0.25%	2.52 ± 0.01***
10 μg/mL/0.25%	2.43 ± 0.02***
25 μg/mL/0.25%	2.20 ± 0.03***
50 μg/mL/0.25%	1.81 ± 0.03***

^aThe nominal starting concentrations of phenanthrene, pyrene, and benzo[a]pyrene were 0.045 μM, 0.03 μM, and 0.005 μM, respectively.

^bThe nominal starting concentration of phenanthrene was 0.035 μM.

^cThe letters d–g denote sample means that differ statistically from one another, p < 0.01.

^***Significantly different from the control mean (0 μg/mL alamethicin/0% methanol), p < 0.01.