Lean male mice (average BW = 30.2 g) received sham or sDVX 4 weeks prior to icvSHU or vehicle infusion for 2 weeks. During that period, the mice were individually housed in sealed chambers. Measurements were taken every 10 min and averaged in bouts of 1 h for energy expenditure (EE) and respiratory exchange ratio (RER). Caloric intake data were accumulated in 2-h intervals.
(A and B) Total (A) and continuous (B) caloric intake.
(C and D) Total (C) and continuous (D) energy expenditure.
(E and F) Average (E) and continuous (F) RER.
Gray vertical bars in (B), (D), and (F) represent the dark phase periods. *p < 0.05, 2-way ANOVA, Sidak post hoc test. Data represent mean ± SEM. n = 8–11.