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Kinetic constants determined for C11-GH1 on azido-modified and unmodified glycosides
| Substrate | Mean kcat (s−1) ± SD |
Mean Km (mM) ± SD |
Mean kcat/Km ratio (mM−1 s−1) ± SD |
|---|---|---|---|
| Gal MU | 0.9 ± 0.2 | 0.05 ± 0.02 | 20 ± 9 |
| 6-N3-Gal MU | 3.9 ± 0.5 | 0.10 ± 0.02 | 40 ± 10 |
| Glc MU | 63 ± 5 | 0.20 ± 0.03 | 320 ± 50 |
| 6-N3-Glc MU | 14 ± 1 | 0.05 ± 0.01 | 280 ± 60 |
