TABLE 2.
Theoretical and sequenced composition of the mock community
| Bacterial species | Sequencing result (%) |
Theoreticala mock community composition (%) |
|---|---|---|
| Lactobacillus fermentum | 22.82 | 18.8 |
| Salmonella enterica | 17.23 | 11.3 |
| Bacillus subtilis | 14.26 | 15.7 |
| Escherichia coli | 11.23 | 10 |
| Staphylococcus aureus | 10.92 | 13.3 |
| Listeria monocytogenes | 10.40 | 15.9 |
| Pseudomonas aeruginosa | 8.11 | 4.6 |
| Enterococcus faecalis | 5.03 | 10.4 |
The theoretical composition was calculated from genomic DNA by the following formula: 16S copy number = [total genomic DNA (g) × unit conversion constant (bp/g)]/[genome size (bp) × 16S copy number per genome].