Fig 3. Mapping-based scaffolding approaches.

(a) Contigs (arrows) are aligned to a reference genome, and their order and orientation is inferred from the alignment. (b) Long reads aligned to the ends of contigs imply their adjacency; (c) optical maps (tics represent location of restriction sites) can be used to infer the order and orientation of contigs (arrows) by aligning the inferred restriction pattern (tics within arrows) to that of the experimental map. contig, contiguous genomic segment.