Table 1. Comparison of different sequencing and mapping technologies.

Category	Scaffolding Data	Separation on the Genome	Orientation	Ordering	Distance
Physical mapping	Restriction maps	10–100 Kb	Yes	No	Yes
	Optical maps	10–100 Kb	Yes	Yes	Yes
Subcloning	10x Genomics	100 Kb	Yes	Yes	Yes
	Illumina TSLR	100 Kbp	Yes	Yes	Yes
Long-read data	Pacific Biosciences	10–15 Kb	Yes	Yes	Yes
	Oxford Nanopore	15–20 Kb	Yes	Yes	Yes
Paired read	Paired-end reads	100–500 bp	Yes	Yes	Yes
	Mate pairs	1,000–10,000 bp	Yes	Yes	Yes
Chromosome conformation	Hi-C	30–100 Mb	Yes	Yes	No
	Chicago	3–100 Mb	Yes	Yes	No
Synteny	Reference genome(s)	Up to genome size	Yes	Yes	Yes

Abbreviation: TSLR, TruSeq Synthetic Long Read.