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. 2019 Jun 5;17(6):e2005326. doi: 10.1371/journal.pbio.2005326

Fig 6. Ngl3−/−(Hyb) and Ngl3−/−(B6) mice display hyperactivity.

Fig 6

(A) Hyperactivity of Ngl3−/−(Hyb) mice (2–4 months) in the open-field test. Data are presented as means ± SEM. n = 11 mice for WT and 13 for KO. *P < 0.05, ***P < 0.001, two-way ANOVA with Bonferroni test and Student t test. (B) Hyperactivity of Ngl3−/−(B6) mice (2–4 months) in the open-field test. Data are presented as means ± SEM. n = 16 mice for WT and 11 for KO. **P < 0.01, ***P < 0.001, ns, not significant, two-way ANOVA with Bonferroni test and Student t test. (C and D) Hyperactivity of Ngl3−/−(Hyb) mice (2–4 months) in the Laboras test, in which mouse movements are continuously monitored for 48 hours. Note that rearing, but not climbing, is increased in Laboras cages, likely reflecting vertical hyperactivity. The 12-hour shades indicate light-off periods. n = 22 mice for WT and 25 for KO, *P < 0.05, **P < 0.01, ***P < 0.001, two-way ANOVA with Bonferroni test and Student t test. (E and F) Normal locomotor activity of Ngl3−/−(B6) mice (2–4 months) in the Laboras test, in which mouse movements are continuously monitored for 48 hours. Note that rearing, but not climbing, is increased in Laboras cages, likely reflecting vertical hyperactivity. n = 13 mice for WT and 8 for KO, *P < 0.05, ns, not significant, two-way ANOVA and Student t test. Primary data can be found in S3 Data. KO, knockout; KO-B, knockout, C57BL/6; KO-H, knockout, hybrid; ns, not significant; WT, wild-type.