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. 2019 May 23;15(5):e1007711. doi: 10.1371/journal.pgen.1007711

Table 2. Genomic regions with Gata4-binding sites assessed by luciferase reporter assay and ChIP-qPCR.

Luciferase assay ChIP-qPCR
Gene name Genomic fragment Locus Luciferase results Gata4-binding sites in subcloned fragments Genomic fragment Locus ChIP results
Gata6 Gata6-luc chr18: 11052898–11054024 1.51±0.97
P = 0.0945
chr18: 11052933–11053086 Gata6-1a chr18: 11051387–11069602 0.02±0.01
P = 0.1128
Gata6-luc/m1 0.90±0.03
P = 0.1198
chr18: 11052933–11052964 Gata6-1b chr18: 11052900–11053090 0.19±0.01
P = 0.0141
Gata6-luc/m2 0.93±0.03
P = 0.3169
chr18: 11053019–11053024 Gata6-2 chr18: 11069461–11069602 0.02±0.00
P = 0.1530
Gata6-luc/m3 0.81±0.05
P = 0.3552
chr18: 11053062–11053066 Gata6-3a chr18: 11074459–11074606 0.01±.01
P = 0.4378
Gata6-3b chr18: 11075106–11075234 0.02±0.01
P = 0.3491
Gata6-3c chr18: 11075344–11075487 0.02±0.00
P = 0.1789
Gata6-4a chr18: 11078180–11078329 0.02±0.01
P = 0.1773
Gata6-4b chr18: 11078433–11078559 0.02±0.01
P = 0.8491
Gata6-ctrl chr18: 11087740–11087888 0.00±0.00
Gli1-ctrl chr10: 126771190–126771322 0.09±0.03

All genomic coordinates are shown in mouse genome build mm9.