Table 2. Genomic regions with Gata4-binding sites assessed by luciferase reporter assay and ChIP-qPCR.
Luciferase assay | ChIP-qPCR | ||||||
---|---|---|---|---|---|---|---|
Gene name | Genomic fragment | Locus | Luciferase results | Gata4-binding sites in subcloned fragments | Genomic fragment | Locus | ChIP results |
Gata6 | Gata6-luc | chr18: 11052898–11054024 | 1.51±0.97 P = 0.0945 |
chr18: 11052933–11053086 | Gata6-1a | chr18: 11051387–11069602 | 0.02±0.01 P = 0.1128 |
Gata6-luc/m1 | 0.90±0.03 P = 0.1198 |
chr18: 11052933–11052964 | Gata6-1b | chr18: 11052900–11053090 | 0.19±0.01 P = 0.0141 |
||
Gata6-luc/m2 | 0.93±0.03 P = 0.3169 |
chr18: 11053019–11053024 | Gata6-2 | chr18: 11069461–11069602 | 0.02±0.00 P = 0.1530 |
||
Gata6-luc/m3 | 0.81±0.05 P = 0.3552 |
chr18: 11053062–11053066 | Gata6-3a | chr18: 11074459–11074606 | 0.01±.01 P = 0.4378 |
||
Gata6-3b | chr18: 11075106–11075234 | 0.02±0.01 P = 0.3491 |
|||||
Gata6-3c | chr18: 11075344–11075487 | 0.02±0.00 P = 0.1789 |
|||||
Gata6-4a | chr18: 11078180–11078329 | 0.02±0.01 P = 0.1773 |
|||||
Gata6-4b | chr18: 11078433–11078559 | 0.02±0.01 P = 0.8491 |
|||||
Gata6-ctrl | chr18: 11087740–11087888 | 0.00±0.00 | |||||
Gli1-ctrl | chr10: 126771190–126771322 | 0.09±0.03 |
All genomic coordinates are shown in mouse genome build mm9.