FIG 4.

Cotreatment with antibiotics and specific antibodies ameliorates lung tissue damage and prolongs the survival of infected mice. (A) A Kaplan-Meier survival plot shows the percentage of survival of mice given the various indicated treatments. Five mice were used for each experimental group. (B) Representative H&E images of lungs from mice administered the various indicated treatments. Combined treatment with antibiotics and anti-cANGPTL4 antibody or with anti-cANGPTL4 antibody and antipneumolysin antibody further improved the lung tissue integrity from single treatment with either antibody or antibiotics. Arrows indicate edema, infiltration of immune cells, and tissue damage patterns. Scale bar = 50 μm. (C) Comparative Gene Ontology results analyzed from RNA sequencing data of lung tissues from infected mice (Flu+S3) administered moxifloxacin (a), anti-cANGPTL4 antibody (b), and a combined treatment with antibiotics and antibodies (c). (D) Representative bright-field images of A549 human alveolar epithelial cells cultured with mock IgG treatment (control), pneumolysin, or pneumolysin with an antipneumolysin antibody at the indicated times posttreatment. Cells were tracked for 48 h using the JuLi Stage system for live imaging. n = 4 independent experiments. Scale bar = 50 μm.