Table 1.
Bacterial strains used in this study.
| Strain | Description | Source |
|---|---|---|
| H. influenzae | ||
| NTHi375 | Wild-type, otitis media clinical isolate | [117] |
| NTHi375ΔhxuCBA-P682 | hxuCBA::spec, SpecR | This study |
| NTHi375ΔsapA-1665 | sapA::ermC::ermC, ErmR | This study |
| NTHi375ΔhbpA-P896 | hbpA::spec, SpecR | This study |
| NTHi375Δhpe-P897 | hpe::spec, SpecR | This study |
| RdKW20 | Laboratory strain, capsule-deficient serotype d | [97] |
| RdKW20ΔhxuCBA-P683 | hxuCBA::spec, SpecR | This study |
| RdKW20ΔsapA-P578 | sapA::ermC::ermC, ErmR | This study |
| RdKW20ΔhbpA-P885 | hbpA::spec, SpecR | This study |
| RdKW20ΔhitBC-P804 | hitBC::ermC, ErmR | This study |
| NTHi3655luxABCDE-P845 | Luminiscent strain derived from isolate NTHi3655. It contains a copy of the luxABCDE operon inserted in the genome. |
[63] |
| E. coli | ||
| TOP10 | Used for cloning assays | Thermofisher Scientific |
| SW102 | Derived from DY380, it contains a defective λ prophage with the recombination proteins exo, bet, and gam being controlled by the temperature-sensitive repressor cI857 |
[75] |