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. 2019 Mar 4;10(1):151–165. doi: 10.1080/21505594.2019.1584027

Figure 2.

Figure 2.

Inhibition of autophagy increases T. cruzi infection in Bcln+/+ mice. C57BL/6J wild type mice (Bcln+/+) were treated with CQ (10 mg/Kg/day) and DFMO (1 mg/g/day) for two days before infection and throughout the experiment as described in materials and methods. (a and e): Parasitemia curves obtained from these animals in comparison with non-treated Bcln+/+ mice. Data were obtained from three independent experiments of at least 4 mice each (Mann-Whitney test, * p < 0.05). (b and f): Survival analysis. Data were obtained from three independent experiments of at least 4 mice each (Log rank Mantel-Cox test) (c and g): Number of amastigotes in cardiac tissue from control and treated mice. Data were obtained from two independent experiments of at least 4 mice each (Mann-Whitney test, * p < 0.05). (d and h): Representative images showing the expression of LC3 forms in the liver of non-infected control or treated Bcln+/+ mice by western blot. Graphics show the OD of LC3-II relative to tubulin expression. Bars indicate the mean ± the standard error of two independent experiments of at least 2 mice each (Student´s t-test, *** p < 0.001).