Figure 2.

RNA binding of wildtype AUF1 p45. (a) FAM-EX-labeled RNA molecules that were applied for binding studies. (b) and (c) Representative binding isotherms with 0.2 nM AU/GU-rich RNA (b) or 25 nM random RNA (c) and increasing concentrations of AUF1 p45. The data were normalized to the initial intensity of the unbound FAM-EX-labeled RNA. The binding curves were fitted to a single-site binding model Equation (1) in materials and methods).