Figure 7. C2-domain sequence alignment for five human cPLA₂ isoforms showing the uniqueness of the Tyr96 residue in the cPLA₂α C2-domain.

β-strand sequences (arrows) as well as CBL2 and CBL3 sequences (bracketed) are shown above the alignment. Green highlights represent identical residues. Cyan highlights represent similar residues. The yellow highlights facilitate comparison of other isoform residues with N65 and Y96 (red) which are key for PC selectivity by the cPLA₂α C2-domain. Underlined black residues in cPLA₂α (R59, R61, and H62) participate in C1P binding (Stahelin et al., 2007; Ward et al., 2013). Sequence alignment was generated using Clustal Omega.