Key resources table.
| Reagent (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | BL21 (DE) Star competent cells | ThermoFisher Scientific | SKU# C6010-03 | Cells for protein expression |
| Transfected construct (E. coli) | pET SUMO | Snapgene |
https://www.snapgene.com/resources/plasmid-files/?set=ta_and_gc_cloning_vectors&plasmid=pET_SUMO
_(linearized) |
Protein expression vector |
| Commercial assay or kit | JCSG Core Suites | Qiagen | https://www.qiagen.com/us/shop/sample-technologies/protein/crystallization/the-jcsg-core-suites/#orderinginformation | Protein crystallization; crystallization screening kit |
| Chemical compound, drug | 1,2-dihexanoyl -sn-glycero-3- phosphocholine |
Avanti Polar Lipids | https://avantilipids.com/product/850305/ | DHPC |
| Chemical compound, drug | 1-palmitoyl-2- oleoyl-glycero-3- phosphocholine |
Avanti Polar Lipids | https://avantilipids.com/product/850457/ | POPC |
| Chemical compound, drug | 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine | Avanti Polar Lipids | https://avantilipids.com/product/840034/ | POPS |
| Chemical compound, drug | 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine | Avanti Polar Lipids | https://avantilipids.com/product/850757 | POPE |
| Chemical compound, drug | 1-palmitoyl-2- oleoyl-sn-glycero-3-phosphate |
Avanti Polar Lipids | https://avantilipids.com/product/840857 | POPA |
| Chemical compound, drug | 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) | Avanti Polar Lipids | https://avantilipids.com/product/840457 | POPG |
| Chemical compound, drug | N-oleoyl-D-erythro-sphingosylphosphorylcholine | Avanti Polar Lipids | https://avantilipids.com/product/860587 | 18:1 SM |
| Chemical compound, drug | 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2–1,3-benzoxadiazol-4-yl) | Avanti Polar Lipids | https://avantilipids.com/product/810145 | NBD-PE |
| Software, algorithm | GeneCards | http://genecards.org | RRID:SCR_002773 | Orthologs; retrieval of protein sequences for human, mouse, and chicken proteins |
| Software, algorithm | UniProtKB | http://www.uniprot.org/help/uniprotkb | RRID:SCR_004426 | C2-domain sequences for various proteins and organisms |
| Software, algorithm | NCBI Protein | http://www.ncbi.nlm.nih.gov/protein | RRID:SCR_003257 | Protein sequences for human, mouse, and chicken proteins |
| Software, algorithm | Clustal Omega | http://www.ebi.ac.uk/Tools/msa/clustalo/ | RRID:SCR_001591 | Software package for multiple sequence alignment |
| Software, algorithm | Clustal W2 |
http://www.ebi.ac.uk/Tools/
msa/clustalw2/ |
RRID:SCR_002909 | Multiple sequence alignment program for DNA or proteins. |
| Software, algorithm | UCSF Chimera | http://plato.cgl.ucsf.edu/chimera/ | RRID:SCR_004097 | Program for interactive visualization and analysis of molecular structures |
| Software, algorithm | Protein Data Bank (PDB) | http://www.wwpdb.org/ | RRID:SCR_006555 | Macromolecular structure archive that oversees and reviews deposition and processing data |
| Software, algorithm | Coot | http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ | RRID:SCR_014222 | Software for macromolecular model building, completion and validation, and protein modeling using X-ray data |
| Software, algorithm | PHENIX | https://www.phenix-online.org/ | RRID:SCR_014224 | Python-based software suite for determination of X-ray crystallographic molecular structures |
| Software, algorithm | PyMol | http://www.pymol.org/ | RRID:SCR_000305 | Data processing, 3D visualization and rendering software |
| Software, algorithm | PDBeFold | http://pdbe.org/fold/ | RRID:SCR_004312 | Co-alignment of compared structures |
| Peptide, recombinant protein | Cytosolic phospholipase A2 | https://www.uniprot.org/uniprot/P47712 | Human cPLA2 sequence | |
| Peptide, recombinant protein | Cytosolic phospholipase A2 |
https://www.uniprot.org/uniprot/P47713 | Mouse cPLA2 sequence | |
| Peptide, recombinant protein | Cytosolic phospholipase A2 | https://www.uniprot.org/uniprot/P49147 | Chicken cPLA2 sequence |