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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Nat Neurosci. 2019 Apr 1;22(5):741–752. doi: 10.1038/s41593-019-0366-7

Fig. 1. Generation of GLT1 promoter reporter mice employing increasing lengths of the DNA sequence upstream of the transcription start site.

Fig. 1

Promoter reporter mice were generated by employing different lengths of the DNA sequences upstream of the transcriptional start site (TSS) of GLT1 followed by tdTomato leading to astroglia-specific tdTomato-expression. (A) Varying length promoter reporter fragments resulted in differential cell-specific tdTomato expression. A fragment of 8.3 kb was required for astroglia-restricted tdTomato expression, whereas fragments ≤ 7.9 kb resulted in neuronal tdTomato expression and no astroglial expression in adults. (B) Coronal section of the 8.3 kb-tdTomato expression. (C) Magnified image of motor cortex representing overlap between 8.3 kb-tdTomato and Glt1-eGFP astrocyte specific expression. (D) No 8.3 kb-tdTomato expression was detected in the hippocampus. N=3 different mice were imaged with analysis of 5 images per mouse.