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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Nat Neurosci. 2019 Apr 1;22(5):741–752. doi: 10.1038/s41593-019-0366-7

Fig. 2. 8.3 kb-tdTomato expression is static and limited to an astroglia subset in the cerebral motor cortex.

Fig. 2

(A) 8.3 kb-tdTomato does not colocalize with neuronal marker, NeuN. (B) 8.3 kb-tdTomato does not colocalize with microglia marker, Iba1. (C) 8.3 kb-tdTomato does not colocalize with myelin and oligodendrocyte marker, CNPase. (D) 8.3-astroglia consist of about 25% of all grey matter astroglia in the motor cortex. (E) 8.3-astroglia are heavily enriched in cortical layers II/III and V. Glt1-eGFP only astroglia are equally distributed across all cortical layers. (F) 8.3 kb-tdTomato nanoparticles are expressed by a subset of GFAP-positive astroglia enriched in layers II/III and V of the mouse motor cortex (i,ii scale bars 15μM). (G) Cortical multiphoton in vivo imaging performed weekly for 5 weeks in adult mice tracking individual 8.3-astroglia (N = 5 mice, 100 cells). Astroglia cell counting was performed with sections imaged and subjected to validated automated cell counting of eGFP and tdTomato fluorescence cells. Statistics used to compare eGFP only to 8.3-astroglia per cortical layer include two-way ANOVA with Tukey post hoc analysis, error bars represent standard error of mean (SEM). p-value statistics used are as following * p< 0.05, ** p< 0.01, *** p< 0.001. For nanoparticle injections, n = 5 mice were intracortically injected and analyzed. For all experiments n = 5 mice were analyzed, 3–5 images per mouse. Red arrows indicate 8.3kb-astroglia and white arrows indicate non-8.3-astroglia cell.