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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Clin Cancer Res. 2019 Feb 15;25(11):3392–3403. doi: 10.1158/1078-0432.CCR-18-1821

Figure 2. Nanostring gene expression analysis reveals AZD6738 significantly modules radiation induced immune-linked transcripts in vivo.

Figure 2

A, Overview of differential gene expression with ATR-radiation combinations in the TC-1 murine model at 5 days after the end of 4 x 2 Gy radiation in the presence or absence of AZD6738. Differential expression analysis of NanoString data was performed, and gene functional annotations used to classify each gene with significantly altered expression into one of the above groups. Genes with significant change in expression (with p < 0.05 by Benjamini-Yekutieli Method) were grouped into functional categories and the number of genes with significant differential expression per category is shown for the indicated pairwise comparisons. Legend indicates which two groups were compared.

B, Venn diagram illustrating numbers of genes with significant changes in expression for the indicated combinations. C, Venn diagram illustrating interferon-stimulated genes, for ATRi-RT combination treatment. The number of genes stimulated by each of type I, II and III interferons is demonstrated. Overall, there is a greater representation of genes that are stimulated by type I interferons in the database, and very few by type III interferons, so this may lead to a degree of bias in these results (from Interferome database).