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Silencing effect of the siRNA–PNA chimeras containing one or two PNAs. The native siRNA or siRNA–PNA chimeras were cotransfected with the firefly luciferase plasmid and renilla luciferase plasmid using Lipofectamine 2000. A scrambled siRNA was used as the negative control. A dual-luciferase reporter (DLR) assay system was used to detect the relative luciferase expression level of each sample. Results are presented as mean ± SD (n = 3 independent experiments).
