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. Author manuscript; available in PMC: 2020 Jun 4.
Published in final edited form as: Structure. 2019 May 2;27(6):1000–1012.e6. doi: 10.1016/j.str.2019.03.022

Figure 6. FAM105A amino-terminus directs its subcellular localization.

Figure 6

Immunofluorescence analysis in Neuro2A cells of FLAG-tagged (A) full-length FAM105A protein, (B) the FAM105A1−100 N-Terminus and (C) the FAM105A84−356 OTU domain. Coimmunostaining for endogenous KDEL (green) marked the endoplasmic reticulum. FLAG-FAM105A and FLAG-FAM105A1−100 largely colocalize with KDEL in the cytoplasm, but FLAG-FAM105A84−356 OTU-domain protein appears diffuse throughout the cytoplasm and in puncta within the nucleus. (D) HA-FAM105A, but not (E) the isolated OTU-domain, HA-FAM105A84−356, localizes to the nuclear envelope, which is marked by immunostaining for Lamin B1 (green). All FAM105A proteins are shown in red.