KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Ub (P4D1)	Covance	MMS-257P
Anti-FLAG (Mouse M2)	Sigma	F1804
Anti-Myc (mouse 9E10)	Santa Cruz	Sc-40
Anti-GFP (mouse)	Roche	11814460001
Anti-HA (rat - high affinity)	Roche	1186742300
Anti-Metl Ub (1E3)	V. Dixit (Genentech)	N/A
Anti-KDEL (mouse MAC 256)	Abcam	Ab50601
Anti-Lamin B1 (rat)	Abcam	Ab16048
Anti-Cy3 rat (donkey)	Jackson Immunoresearch	712-165-150
Anti-Cy3 mouse (donkey)	Jackson Immunoresearch	715-165-150
Anti-mouse AlexaFluor 488 (donkey)	Invitrogen	A-21202
Anti-rat AlexaFluor 488 (donkey)	Invitrogen	A-21208
Bacterial and Virus Strains
BL21(DE3) chemically competent E.coli	Thermo Fisher	C600003
Chemicals, Peptides, and Recombinant Proteins
Linear diUbiquitin	Boston Biochem.	UC-700
K6 diUbiquitin	Life Sensors	SI0602
K11 diUbiquitin	Boston Biochem.	UC-40
K27 diUbiquitin	Boston Biochem.	UC-61B
K29 diUbiquitin	Boston Biochem.	UC-81
K33 diUbiquitin	Boston Biochem.	UC-101
K48 diUbiquitin	Boston Biochem.	UC-200
K63 diUbiquitin	Boston Biochem.	UC-300
Ub-AMC	Boston Biochem.	U-550
Ubiquitin	Rivkin et.al, 2013	N/A
Sypro Orange	Thermo Fisher	S6650
15N-Ammonium Chloride	Cambridge Isotope	NLM-467–50
Effectene transfection reagent	Qiagen	301425
Protease inhibitor - Complete mini EDTA free	Sigma-Aldrich	11836170001
Protein A/G plus agarose beads	Santa Cruz	SC-2003
Paraformaldehyde	Sigma-Aldrich	P6148
4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI)	Vector Laboratories	H-1200–10
D-Biotin	Biobasic	BB0078
Mammalian protease inhibitor cocktail	Sigma-Aldrich	P8340
Streptavidin immobilized to Sepharose	GE	17-5113-01
Sequencing grade modified trypsin	Promega	V5113
T etracycline	Sigma-Aldrich	T7660
Turbonuclease	Sigma-Aldrich	T4330–50KU
TPCK trypsin	Promega	V-5111
USP2	Ernst et al., 2012	N/A
Critical Commercial Assays
Protein Thermal Melt Assay	Roche	LightCycler 480
Multiwell microplates	Roche	04 729 749 001
Dual luciferase assays	Promega	E1910
Deposited Data
Crystal structure of FAM105A 87–356	This paper	PDB: 6DRM
Raw mass spectrometry data archive	This paper	MassIVE: MSV000082315
Crystal structure of OTULIN 79–352	Rivkin et al., 2013	PDB: 4KSJ
Crystal structure of OTULIN and M1-diUb	Rivkin et al., 2013	PDB: 4KSK
Crystal structure of OTULIN and M1-diUb	Keusekotten et al., 2014	PDB: 3ZNZ
Experimental Models: Cell Lines
Neuro2A	Rivkin et al., 2013	N/A
HEK293T	Rivkin et al., 2013	N/A
Flp-In T-REx 293	ThermoFisher	R78007
Oligonucleotides
FAM105A-N87: cgggatccaacctcagtgtggaggcagag	Millipore Sigma	N/A
FAM105A-C356: ccgctcgagttaaaagactggaatgtggtagtggcg	Millipore Sigma	N/A
D139C: cgacaagtaaggagagataactatTGT gctctcagatcagtgttatttcag	Millipore Sigma	N/A
H352N: ctgaccgagaacgaccgccactacAACattccagtcttttaactcgaggc	Millipore Sigma	N/A
SUMO 1-N1: gcggatccatgtctgaccaggaggcaaaacc	Millipore Sigma	N/A
SUMO 1-C97: ccgctcgagttaaccccccgtttgttcctg	Millipore Sigma	N/A
SUMO2-N1: cgggatccatggccgacgaaaagcccaaggaagg	Millipore Sigma	N/A
SUMO2-C93: ccgctcgagttaacctcccgtctgttggaacac	Millipore Sigma	N/A
ATG8-N1: gcggatccatgaagttccagtacaaggaggac	Millipore Sigma	N/A
ATG8-C116: ccgctcgagttacccatagacactctcatcactgtaggc	Millipore Sigma	N/A
ISG15-N1: cgggatccatgggctgggacctgacggtg	Millipore Sigma	N/A
ISG15-C157: ggcctcgagctagcctccccgcaggcgcag	Millipore Sigma	N/A
FAT 10-N8: cgggatccctctgtgtgcatgtccgttccgag	Millipore Sigma	N/A
FAT 10-C82: ccgctcgagttacttcaccactttcagggtaaggtg	Millipore Sigma	N/A
FAT10-N82: cgggatccaagcccagtgatgaggagctgccc	Millipore Sigma	N/A
FAT 10-C165: ccgctcgagtcaccctccaatgcaataacatgccag	Millipore Sigma	N/A
Ufm1-N1: cgggatccatgtcgaaggtttcctttaagatcacgc	Millipore Sigma	N/A
Ufm1-C83: ccgctcgagttatccaacacgatctctaggaataatccgc	Millipore Sigma	N/A
FAM105A-WT-F: aggcgcgccaatggcggcgacaaggagccccacg	Millipore Sigma	N/A
FAM105A-M84-F: aggcgcgccattcaaaaggaacctcagtgtggag	Millipore Sigma	N/A
FAM105A-C100X-R: cactcgagtcaacaataactgagtaaatcaacctc	Millipore Sigma	N/A
FAM105A-WT-R: cactcgaggctaaaagactggaatgtggtagtggcggtc	Millipore Sigma	N/A
GW-FAM105A-C100-R: ggggaccactttgtacaagaaagctgggtaacaataactgagtaaatcaacctc	Millipore Sigma	N/A
GW-FAM105A-F1: ggggacaagtttgtacaaaaaagcaggctggagacatggcggcgacaaggagccccacg	Millipore Sigma	N/A
GW-FAM105A-R1: ggggaccactttgtacaagaaagctgggtaaaagactggaatgtggtagtggcggtc	Millipore Sigma	N/A
Recombinant DNA
ProEx FAM105A - human 87–356	This paper	BC011524
ProEx AT G8 - human 1–116	This paper	BC009309
ProEx ISG15 - human 1–157	This paper	DQ892714
ProEx FAT10 - human 8–82, 82–165	This paper	BC012472
ProEx SUMO1 - human 1–97	This paper	BC053528
ProEx SUMO2 - human 1–93	This paper	BC008450
ProEx UFM1 - human 1–83	This paper	BC005193
pGEX-Ub	Rivkin et.al, 2013	N/A
PRoEx-linear diUb	Rivkin et.al, 2013	N/A
HA-FAM105A 1–356	This paper	BC011524
HA-FAM105A 84–356	This paper	BC011524
FLAG-FAM105A 1–356	This paper	BC011524
FLAG-FAM105A 1–100	This paper	BC011524
FLAG-FAM105A 84–356	This paper	BC011524
FLAG-OTULIN	Rivkin et al, 2013	N/A
FLAG-OTULIN C93S	Rivkin et al, 2013	N/A
HA-HOIL	Rivkin et al, 2013	N/A
Myc-HOIP	Rivkin et al, 2013	N/A
Myc-MAPKK 1-R4F	Mansour et al, 1994	http:/n2t.net/addgene:40810
pcDNA5 FRT/TO FAM105A	This paper	BC011524
pcDNA5 FRT/TO CALR3	This paper	BC014595
pcDNA5 FRT/TO SYVN1	This paper	BC030530
Software and Algorithms
Adobe Photoshop	Adobe	CS6
Xia2	Winter et al., 2013	0.5.542
XDS	Kabsch et al., 2010	20170923
Phaser	McCoy et al., 2007	2.5.2
PHENIX	Adams et al., 2011	1.13
COOT	Emsley et al., 2004	0.8.9.1
NMRPipe/NMRDraw	Delagio et al., 1995	20180515
NMRView	Johnson et al., 2004	8.0.3
Light Cycler Protein Melting software	Roche	N/A
Excel	MicroSoft	16.16.5
ProHits	Guomin et al., 2010	5.0.2
Proteowizard	Kessner et al., 2008	v.3
X!Tandem	Craig and Beavis, 2004	Alanine 2017.02.01
Comet	Eng et al., 2013	2018011
SAINTexpress	Teo et al., 2014	3.6.1
ToppGene	Chen et al., 2009	https://toppgene.cchmc.org/