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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: DNA Repair (Amst). 2019 Apr 27;79:1–9. doi: 10.1016/j.dnarep.2019.04.006

Figure 2.

Figure 2.

Characterization of HBECs deficient in DNA-PKc expression. (A) Levels of DNA-PKc expression in selected clones relative to p-Silencer and normalized to GAPDH. (B) Western blot showing reduced DNA-PKc expression in HBEC clones with gene knockdown compared to p-Sil. (C) DNA Repair capacity is decreased in HBECs with knockdown of DNA-PKc. HBECs were treated with 0.25U/L bleomycin for 1 hour, and subsequently with cytochalasin for 48 hrs. Cells were harvested and analyzed for DRC measured by the cytokinesis-block micronucleus assay. (D) DNA-PKc deficiency enhances transformation of HBECs following treatment with bleomycin once a week for 12 weeks. Error bars are the mean ±SD and p<0.05 using a paired, two-tailed t test.