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. Author manuscript; available in PMC: 2020 Jun 4.
Published in final edited form as: Structure. 2019 Apr 11;27(6):937–951.e5. doi: 10.1016/j.str.2019.03.012

Figure 6. Ubiquitin binding disassembles mutant UBQLN2 droplets and aggregates.

Figure 6.

(A) DIC microscopy of UBQLN2 droplet disassembly as ubiquitin is added. Ubiquitin was allowed to diffuse into the sample using channel slides. Ubiquitin was added in 1:1 molar stoichiometry with indicated protein at 100 μM protein in buffer containing 20 mM NaPhosphate and 200 mM NaCl (pH 6.8). Scale bar = 5 μm. (B) Spectrophotometric turbidity assay as a function of temperature for mixtures of Ub and UBQLN2 at indicated ratios. Assays used 25 μM UBQLN2 protein. (C) Chemical shift perturbations (CSPs) for residues in UBQLN2 450–624 at the titration endpoint with Ub.