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. 2019 Jun 5;10(6):444. doi: 10.1038/s41419-019-1681-3

Fig. 3. Verify the effect of ZNF384 on the proliferation ability of hepatocellular carcinoma cells by knocking-out ZNF384 through Crispr/CAS9.

Fig. 3

a The detail information of the two sgRNAs we chose. b Western blot analysis of ZNF384 expression in Huh7 cells treated with Crispr/CAS9 targeting ZNF384. c Knocking-out ZNF384 expression in Huh7 cells effectively inhibits cell growth. e, f Knocking-out ZNF384 expression in Huh7 cells effectively inhibits G1/S phase transition through Flow cytometry analysis. All results are expressed as the mean ± SD of three independent experiments (*0.01 ≤ P < 0.05; **0.001 ≤ P < 0.01, ***P < 0.001)