Fig. 6. ZNF384 binds to the promoter region of cyclinD1 to regulate the expression of cyclin D1.

a Western blotting shows that the expression of Cyclin D1 was down-regulated in both of shZNF384-transfected and sgRNA-transfected cells compared with empty vector-transfected cells. b Western blotting shows that the expression of Cyclin D1 recovered when we restored ZNF384 expression in sgRNA-transfected cells. c The expression of ZNF384 and Cyclin D1 in 60 HCC samples through IHC. d The correlation between the expression of ZNF384 and Cyclin D1 in correlation analysis (n = 60, part of dots was overlapped). e The structure of the luciferase plasmid in this study. f ZNF384 recognizes and bound to the promoter region of Cyclin D1 and regulates Cyclin D1 transcription. g Binding of ZNF384-Flag to the predicted combining capacity in Cyclin D1 promoter region by ChIP assays. Input DNA was used as positive control and ddH₂O was used as a negative control