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. 2019 Jun 5;10(6):444. doi: 10.1038/s41419-019-1681-3

Table 2.

Detail information for the primers we used. F, forward; R: reverse

Name Sequences (5′-3′)
sgRNA-1 F: CACCGGCCTCAGTGTCCCTGCCCTC
sgRNA-1 R: AAACGAGGGCAGGGACACTGAGGCC
sgRNA-2 F: CACCGGCCAGAGAAGGGCTGTGGTC
sgRNA-2 R: AAACGACCACAGCCCTTCTCTGGCC
XbaI-ZNF384 F: GCCTCTAGAATGGAAGAATCTCACTTCA
BamHI-ZNF384 R: TTTGGATCCCTAAGAGCTGGCCAGGTGC
NheI-CCND1 F: CTAGCTAGCCACCCCCAACAAAACCAATT
HindIII-CCND1 R: CCCAAGCTTTTCCTACCTTGACCAGTCGG
Mutate-CCND1 F: ATTTCTTTTTTAATTAAACGGAATGAGTCAG
Mutate-CCND1 R: GATCTCCATTCTGACTCATTCCGTTTAATTA