Fig. 2.

SOX6 was detected as a target of miR-499-5p. a The binding site of SOX6 3′-UTR with miR-499-5p. b The luciferase activity of reporter vector with WT or MUT SOX6 3′–UTR in cells (^**P < 0.01 vs. blank). c The mRNA expressions of miR-499-5p in control, mock, mimic and inhibitor group were determined by RT-qPCR. d The mRNA expressions of SOX6 in control, mock, mimic and inhibitor group were detected by RT-qPCR. (E, F) The SOX6 protein levels in control, mock, mimic and inhibitor group were determined by western blot. GAPDH was as an internal reference. ^**P < 0.01 vs. control, ^{^^}P < 0.01 vs. mock