Figure 6.

(A) Representative H&E images of samples from US DeCal and Normal DeCal groups. The transverse sections showed numerous osteons with peripheral cement line. Well-stained osteocyte nuclei indicate that the decalcification endpoint was not exceeded. There is no significant difference between the two groups with respect to the histological features and presence of artefacts; (B) The ratio of the osteocytes with identifiable nucleus to the total number of osteocytes did not show any significant difference between US DeCal and Normal DeCal groups. Magnification: 200×. Scale Bar: 200μm.

H&E = haematoxylin and eosin; Normal DeCal = normal decalcification group; US DeCal = Ultrasound decalcification group.