Figure 6.

Effect of serum deprivation and BJ on intracellular ROS production, morphology and cell viability in MSCs. MSCs were cultured for 24 h in growth medium with 10%, 0.5%, 0.1% or no FBS, and treated or not with various concentrations of total soluble polyphenols (TSP) of BJ, as reported in Materials and methods. (A) Intracellular ROS production was detected as described in Figure 1 and the data are expressed as fold‐increase over values obtained in cells cultured in 10% FBS. (B) Morphological images of MSCs representative of at least three independent experiments; scale bar: 25 μm. (C) Cell viability was detected by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐tetrazolium bromide (MTT) and the absorbance values are reported as arbitrary units (A.U.) from 10⁴ cells. The data are the mean ± SEM of three experiments performed in triplicate. Data were evaluated by using one‐way ANOVA followed by Bonferroni's post hoc test. *P ≤ 0.05 compared to cells grown in 10% FBS; ^○ P ≤ 0.05 compared to cells grown in 0.5% or 0.1% FBS without BJ TSP.