Table 5.

ANOVA of quadratic model for the EE%, DL%, particle size and zeta potential responses

Factors	Y1		Y2		Y3		Y4
Coefficient	P-value	Coefficient	P-value	Coefficient	P-value	Coefficient	P-value
Model	-	<0.05*	-	<0.05*	-	<0.01**	-	<0.01**
X1	−1.84	0.34	−0.04	<0.01**	12.38	<0.01**	−4.67	<0.0001***
X2	−8.18	<0.01**	−0.03	<0.05*	−3.02	0.13	0.24	0.65
X1X2	−2.9	0.29	<0.01	0.38	−2.9	0.27	1.15	0.15
X12	−5.05	<0.05*	−0.01	0.18	0.59	0.76	1.61	<0.05*
X22	−7.78	<0.01**	−0.01	0.18	0.42	0.83	1.41	<0.05*
R2	0.8764		0.8692		0.9057		0.9466

Notes: The significance of the effect of independent variables on responses: *P<0.05; **P<0.01; ***P<0.0001.

Abbreviations: X₁, oil/mixed-surfactants ratio; X₂, proportion of aqueous phase; Y₁, encapsulation efficiency (%); Y₂, drug loading (%); Y₃, particle size (nm); Y₄, zeta potential (mV).