Figure 3. Loss of Mael does not alter Heterochromatin.

(A) ChIP-seq was used to measure the change between mael^M391/r20 and control ovaries in the density of H3K9me3, HP1a, or Rhi for transposons between mael^M391/r20 or w¹¹¹⁸ control ovaries. Hashed grey line, ≥2-fold change. Transposons were classified according to Wang et al. (Wang et al., 2015). Data are for uniquely mapping reads from the mean of two biological samples.

(B) ChIP-seq was used to measure the change between mael^M391/r20 and control ovaries in the density of H3K9me3, HP1a, or Rhi for piRNA clusters between mael^M391/r20 or w¹¹¹⁸ control ovaries. Hashed grey line, ≥2-fold change. Data are for uniquely mapping reads from the mean of two biological samples.

(C) ChIP-seq was used to measure the change between mael^M391/r20 and control ovaries in the density of H3K9me3, HP1a, or Rhi for piRNA clusters, transposons, and protein-coding genes. Circle denotes the median. Left and right whiskers encompass the first to third quartiles. Density was calculated using a 1 kbp sliding window with a 500 bp step and was normalized to the total number of reads mapping to each chromosome arm. Data are for uniquely mapping reads from two biological samples.