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. 2019 May 27;12:801–811. doi: 10.2147/DMSO.S206558

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© 2019 Liu et al.

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MCC950 inhibits SREBP-1c expression and prevents its regulation on downstream target genes. (A) Metabolism-related signaling pathway was detected by Western blot in fasted and refed mice challenged with MCC950 or vehicle, with β-tubulin as a loading control. (B) Quantitative results of the protein levels of mature or precursors SREBP-1c, Casp1, IL1β. * P˂0.05, ** P˂0.01 and *** P˂0.001 compared to the fasted group treated with vehicle, ^# P˂0.05 and ^###P˂0.001 compared to the vehicle group under the same diet treatment. (C) Normalized activities of the luciferase reporters analyzing the effect of MCC950 on SREBP-1c promoter activation which was induced by PA in HepaRG cells. ^& P<0.05 and ^&& P<0.01. (D) Chromatin immunoprecipitation (ChIP) assay using chromatin isolated from livers of mice after fasting or refeeding. Nuclear extracts were immunoprecipitated with an anti-SREBP-1c antibody, and purified DNA was subjected to qPCR by specific primers located at the promoter region of mouse FASN and ACC1. ^# P˂0.05 compared to the refed group treated with vehicle.