Figure 5.

Ischemia duration correlates with early and late mRNA expression of injury biomarkers. To determine the effect of ischemia time on expression of KIM-1 and NGAL, mice underwent unilateral left ischemia or sham procedure for either 32 or 60 minutes. The left kidneys were collected after euthanasia at 2, 6, and 24 hours, and 14 and 28 days after the procedure, and (A) KIM-1 and (B) NGAL were measured via RT-PCR. n=3–10; P<0.05. * indicates a statistically significant difference between either the 32 or 60 minute sham and UiAKI groups, ** indicates a statistically significant difference between the 32 and 60 minute UiAKI groups.