Figure 4.

sns and c3g genetically interact in nephrocytes. (A) Immunofluorescence analysis of nephrocytes overexpressing sns or control plasmid by sns-Gal4. Nephrocytes were stained with antibodies specific for Integrin β (green) and Sns (red). Merged images and higher magnifications (×4) of the marked area are shown in the columns to the right. Scale bar, 10 µm. (B) Shown are TEM images from Drosophila with overexpression of sns and knockdown of the control gene or83b, or flies with sns overexpression and c3g knockdown in nephrocytes. Knockdown in nephrocytes was accomplished using sns-Gal4. Scale bar, 200 nm. n=3.