Figure 6.

Localization of asialo-podocalyxin is preserved in vivo, whereas nephrin appears mislocalized along with other SD components at P28 and dramatically reduced at P42. (A) Analysis of the localization of nephrin (red) and podocalyxin (green) in control and PCmas^−/− mice by indirect immunofluorescent staining on sections of paraffin-embedded renal tissue sampled at P21, P28, and P42. Sections were costained with specific antibodies against nephrin and podocalyxin and visualized with fluorophore-conjugated secondary antibody. Nuclei were stained with DAPI. (B) Indirect immunofluorescent costaining of nephrin (red) with SD proteins neph1 and ZO-1 (green) on paraffin-embedded and podocin (green) on frozen kidney sections of control and PCmas^−/− mice sampled at P28.