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. 2017 Nov 2;1(2):90–106. doi: 10.15698/cst2017.11.111

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Copyright: © 2017 Grau et al.

This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged.

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Confocal microscopy of DAPI stained (blue) HEK293T cells expressing tested homo-dimers (17L [VN-17L/VC-17L], 19L [VN-19L/VC-19L], 23L [VN-23L/VC-23L], 25L [VN-25L/VC-25L], 27L [VN-27L/VC-27L], 29L [VN-29L/VC-29L]). TM-driven homo-oligomerization results in VFP reconstitution and fluorescent signal (green). Sec61α fused to mCherry fluorescent protein was used as ER marker (red). The presence of colocalization of red and green signals in the merge images was highlighted in yellow. Scale bar size was set to 16 μm.