Fig. 3.

Over-expression of miR-195-5p represses angiogenesis and reduces chemotherapy resistance in OC. a, b Protein expression of HIF-1α and VEGF in adjacent normal tissues (n = 25) and OC tissues (n = 77) detected by Western blot analysis; *p < 0.05 vs. adjacent normal tissues; c expression of miR-195-5p in SKOV-3 cells after transfected with corresponding sequences detected by RT-qPCR; *p < 0.05 vs. the mimic NC group; ^#p < 0.05 vs. the inhibitor NC group. d, e Protein expression of HIF-1α and VEGF in SKOV-3 cells transfected with miR-195-5p mimic or inhibitor measured by Western blot analysis; *p < 0.05 vs. the mimic NC group; ^#p < 0.05 vs. the inhibitor NC group; f inhibition rate of SKOV-3 cells at different DDP concentrations after transfection with miR-195-5p mimic or inhibitor evaluated by CCK-8; *p < 0.05 vs. the mimic NC group; g IC₅₀ value of DDP in SKOV-3 cells after transfected with corresponding sequences detected by CCK-8; *p < 0.05 vs. the mimic NC group; h, i apoptosis of SKOV-3 cells after transfection with miR-195-5p mimic or inhibitor measured by flow cytometry; *p < 0.05 vs. the mimic NC + saline group; ^#p < 0.05 vs. the mimic NC + DDP group. The above results are measurement data, and expressed as mean ± standard deviation; unpaired t-test is used to analyze the data between two groups; while one-way ANOVA is used to analyze the data among multiple groups; the repeated measures ANOVA is performed at different time points; the experiment was independently repeated three times