Fig. 6.

Over-expression of miR-195-5p restrains angiogenesis via regulating PSAT1 and GSK3β/β-catenin signaling pathway in vivo. a, b Protein expression of PSAT1, GSK3β, β-catenin, HIF-1α and VEGF as well as the extent of GSK3β phosphorylation in xenograft tumor of nude mice detected by Western blot analysis; *p < 0.05 vs. the agomir NC group; c immunohistochemical staining results in xenograft tumor in nude mice (×400, scale bar = 25 μm). d MVD in xenograft tumor in nude mice; *p < 0.05 vs. the agomir NC group. The figure data are measurement data and represented as mean ± standard deviation; one-way ANOVA is used among multiple groups and t-test is used between two groups; the experiment was independently repeated three times