Table 2.

Effect of cellulase recycling on the overall glucose yields and total amounts of produced glucose (mean ± standard deviation) from the enzymatic hydrolysis of hydrothermally-pretreated EFBs

Mode of  the two-round enzymatic hydrolysis	Hydrolysis round	Cellulase loading (FPU/g glucan)	Glucose yield (% of theoretical max.)	Total amount of glucose produced (g)c
Separate hydrolysis without cellulase recycling	R1a	20	78.2 ± 0.1	3.70 ± 0.00
	R2b	20	78.2 ± 0.1
Cellulase recycling	R1a	40	98.7 ± 0.8	2.79 ± 0.15
	R2b	0	19.3 ± 5.7
Cellulase recycling with the additional treatments	R1a	40	98.7 ± 0.8	3.94 ± 0.06
	R2b	0	68.0 ± 1.8

^aThe first round of enzymatic hydrolysis

^bThe second round of enzymatic hydrolysis

^cThe overall glucose produced from 4 g of pretreated EFBs at the first round and 4 g of pretreated EFBs at the second round